One of the applications of Sony SH800S Cell Sorter is how to sort fluorescent protein expressing cells clearly and with high purity and viability. This application note shows how you can easily sort fluorescent protein expressing cells with the SH800S sorter from Sony.
Fluorescent proteins such as GFP, YFP, CFP and RFP are used as reporter molecules in a variety of studies analyzing protein expression. Such cells have a broad dynamic range of expression and need highly sensitive optical systems for detection.
The Sony sorter is a compact bench sorter completely automatized for set-up, acquisition, sort and analysis. This advanced automation, guaranteed also by a novel microfluidic sorting chip patented technology, allows routine sorting such as the rapid separation of fluorescent protein expressing cells post transfection. Thanks to the automation and ease to use of this instrument you can sort in complete autonomy, for example avoiding the long times booking in the facs facility of your institute.
The quality of the Sony optical system also allows the signals deriving from spectrally adjacent fluorescent proteins to be clearly separated. Particularly, this Sony Note shows the perfect separation for example between GFP and YFP expressing cells, two fluorescent proteins with high spectral overlap, with the help of compensation wizard of SH800S software; moreover, post sorter check analysis displays also the good ability of this instruments to deliver cells with high purity and viability.
This application note wants to demonstrate how you can sort fluorescent protein expressing cells quickly and clearly separated without important sorting skills.