The MA900 from Sony meets the needs of most sorting applications, supporting 12 fluorescence parameters and 4-way sorting.
Powerful, modern technologies built into the MA900 system include a patented micro fluidic chip-based design, comprehensive fluidic controls, and advanced automation that dramatically simplifies operation to make sorting less subjective and improve reliability.
Up to 14 parameters from 4 lasers
The MA900 offers choice and flexibility, enabling the detection of up to 14 parameters. Choose from 4 excitation lasers—488 nm, 405 nm, 561 nm, and 638 nm—on two beam spots. Free-form PMTs enable detection of fluorescence signals from each beam spot, allowing detection of up to 12 fluorescence parameters and two scatter parameters.
Startup automation ensures optimal daily alignment of the chip to lasers
With the highest level of automation available on any cell sorter, the MA900 uses sensors, software, and engineering know-how across the workflow from startup to aseptic cleaning, QC, and sort setup. Intelligent automation dramatically simplifies operation and streamlines troubleshooting.
Upon startup, the software initiates connection between all subsystems and runs diagnostics that ensure that everything is working properly. Once the subsystems are verified, the software displays status and a green ready message on the instrument’s LCD monitor.
Automated Chip Loading and Positioning
System setup begins with a one-touch installation to load the microfluidic flow cell chip. A setup software wizard guides you through the process of loading a sorting chip, selecting lasers, and inserting the optical filters required. Actuators ensure precise positioning of the chip inside the chip loader. The microfluidic sorting chip uses patented CoreFinder™ technology to automate and streamline key steps in the workflow including chip alignment and accurate calculation of the laser delay, drop delay, side stream angle, and breakoff position. Once the chip is loaded, the fluidics check starts, and sheath, sample, and vacuum lines connect and seal automatically to their respective ports.
Automated Optical Axis Adjustment
Automated Droplet Calibration
Automated Side Stream Calibration
Automated Quality Control
Using QC beads, the rCV and linearity are measured and can be displayed in Levey-Jennings plots. This information helps administrators accurately assess optical performance over time.
Automated Drop Delay Calibration
Automated Fluid Sensing
Wizard-Driven Cleaning Cycle
Sorting that adapts to your needs with default and custom sort modes as well as 4-way sorting
Once events are gated on plots, target cells can be sorted for further analysis. Sort mode, sort gates, and sort devices can be assigned using a simple dialog box. A choice of eight default and five custom sort modes is available to achieve the desired purity and yield of the sorted population. Sort devices supported include 2-way and 4-way tubes as well as 6-, 12-, 24-, 48-, 96-, and 384-well plates.
Experiments can be created by selecting a new template, a recent experiment, or a shared template (public) from the experiment window. If a new template is selected, the software guides you through choosing experiment settings such as sample groups, tubes, and pulse parameters for data acquisition. Once a template is selected or created, compensation data can be acquired easily by using the software based compensation wizard. Alternatively, uncompensated populations can be adjusted using the drag and drop feature in the software.
Data Display and Using Gates
During acquisition, data is displayed on worksheets as dot plots and histograms. Events in the plots can be marked using gates. A variety of tools are provided to select, adjust, label, and measure statistics of target populations. Once acquired, data can be easily exported as FCS formats to use with third party analysis software.
Automated Sort Monitoring.
he software monitors and actively makes adjustments to the drop drive to maintain a stable breakoff point. This feature ensures consistently good sort performance, facilitates walk-away operation, and allows detection of clogs and empty tubes.
Sort Deposition System
The Sort Deposition System is an optional hardware feature that facilitates high-throughput sorting and precise deposition of cells into 6-, 12-, 24-, 48-, 96-, and 384-well plates or PCR plates. Several features on the MA900 enable deposition of single cells at a high efficiency. These include the ability to precisely adjust the position of a plate, assign the center of the drop relative to the target cell position, and utilize custom angled plate holders for multiwell PCR and 384-well plates.
Index Sorting Software
Index sorting software records the X and Y coordinates of each event sorted into a multiwell device. This very precise and easy to use software brings powerful capabilities to research, enabling you to track the scatter and fluorescence intensity of individual cells sorted in each well.
After the sort, the index file can be exported in CSV format or analyzed on the cell sorter. The Well Select feature lets you choose a well and display event(s) sorted in that well on bivariate plots and histograms. The Gate Select feature lets you display the location of events on a plate map based on a selected gate.
These index sort options allow you to perform meta-analysis of data for several applications. For example, clonal variability can be studied based on the expression levels of the fluorescent protein or surface markers. Also, researchers can integrate phenotypic data with mRNA expression analysis of the sorted cells.
Discover MA900 Multi-Application Cell Sorter applications:
Scatter was used for gating lymphocytes (A). The CD3+ population (B) was used to gate CD4+ and CD8+ cells (C). CD4+ T-cell subsets were identified based on CD45RA and CD27 expression (D). CD16+CD56+ NK cells were gated from CD3- cells (E). CD19+CD20+ B cells were gated from CD3- cells (F), and the HLA-DR expression of B cells was analyzed (G). CD14+ monocytes were identified based on scatter (H). CD3+ T cells, CD19+CD20+ B cells, CD16+CD56+ NK cells, and CD14+ monocytes were sorted by 4-way sorting. Post-sort analysis of each sorted population is shown (I–L
The CD3+ population (B) was used for gating CD4+ and CD8+ cells (C). CCR7+CD45RA+ lymphocytes are shown in (D). CD19+CD20+ B cells were gated from lymphocytes (E), and the HLA-DR expression of B cells was analyzed (F). CD16+ NK cells (G) were gated from lymphocytes. CD27 expression of CD45RA+ subsets of lymphocytes was analyzed (H).
Using the 4-way sort mode on the MA900 cell sorter, CD4+ and CD8+ T cells, CD19+ B cells, and CD16+ NK cells were sorted. Post-sort analysis of each sorted population is shown (G–J).
MA900 Multi-Application Cell Sorter Options
If desired, the MA900 can be installed inside an optional custom class A2 level II biosafety cabinet for protection for personnel and products. The custom biosafety cabinets offered by Sony Biotechnology are designed and tested by their manufacturers using microbiological assays with the MA900 in the work area. The cabinets meet international standards including the National Sanitation Foundation Standard 49 (NSF49) and the European Standard 12469.
The cabinets incorporate a built-in aerosol management system which operates independently to actively evacuate aerosols from the sort collection chamber. The dual routes of aerosol evacuation maximize protection.
MA900 Multi-Application Cell Sorter Specifications