SH800S Cell Sorter: Sorting Made Simple™
The benchtop SH800S cell sorter permits sorting of a wide range of cell sizes for many applications using the 70-μm, 100-μm, and 130-μm microfluidic sorting chips. This novel, chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective, more precise and easier to perform.
System software is intuitive and supports sorting into tubes and 96- and 384-well plates. The software generates FCS 3.0 and FCS 3.1 files that can be exported to third party analysis tools. The SH800S is for non-clinical research use only and is a Class 1 laser product.
The SH800S is flexible and can support a variety of applications for individual and core labs. For applications in which cross-contamination is a concern, optional e-beam sterilized consumables can be used to replace the sample line and sorting chip. An optional class A2 Level II biosafety cabinet provides protection for personnel and products. The biosafety cabinet was custom designed for the SH800S and tested by The Baker Company to meet international standards. The optical design offers up to four collinear excitation lasers (488 nm, 405nm, 561nm and 638nm) and six fluorescence detectors. The six free-form PMTs enable detection of fluorescence signals from any laser based on filter selection.
Automation from Set-up to Analysis
The SH800S Cell Sorter provides the highest level of automation and ease of use available in a cell sorter. Researchers can accurately set up, calibrate, and monitor sorting with a push of a button. This delivers true ease of operation to save time and improve consistency of results.
A novel microfluidic sorting chip uses patented CoreFinder™ technology to automate key steps of instrument setup and operation, streamlining workflow.
The system is versatile, allowing researchers to sort of a wide range of cell sizes to accommodate application requirements. Chip sizes include 70 μm, 100 μm, and 130 μm.
Automated Chip Loading and Positioning
Automated Optical Axis Adjustment
Automated Side Stream Calibration
Automated Drop Delay Calibration
Automated Sort Monitoring and Droplet Calibration
The SH800S Cell Sorter software monitors and actively makes adjustments to the drop drive to maintain a stable breakoff point. This feature ensures consistently good sort performance and allows detection of clogs and empty tubes, and facilitates walk-away operation.
The droplets are automatically calibrated by adjusting the frequency and the drop drive to achieve optimal break off point (BOP) for each type of sorting chip.
Software, sensors, and CoreFinder patented technology provide automation across the workflow from set-up to shutdown to ensure consistency, save time and improve the accuracy of results. The interface is designed with ribbon tabs that logically organize features to make them rapidly accessible. An experiment-centric approach makes the software easy to teach, learn, and use.
Software wizards include step-wise workflows that guide users through start up, multicolor compensation, sorting, and system cleaning. Since the system simplifies even advanced sorting techniques, it can be used by researchers with little sorting experience.
Upon start up, the system initialization includes diagnostics that ensure all subsystems are properly working. Once verified, the system status and a green ready message are displayed on the LCD monitor on the front of the instrument.
Initial Instrument Set-up, Precision Alignment, and Calibrations
The set up wizard guides researchers through the process of loading a sorting chip, selecting lasers, and inserting the optical filters required.
When complete, the fluidics check starts, and sheath fluid droplets appear from the tip of the sample probe. If needed, de-bubble and sample line cleaning wizards can be run. Once complete, the auto calibration screen is displayed.
Alignment and Calibrations
The system automatically and precisely aligns the sorting chip to the lasers, calibrates the droplets, and side streams and estimates the drop delay using AutoSetup beads.
Users can create an experiment by selecting a new template, a recent experiment, or a shared template (public) from the Experiment window. If a new template is selected, dialogs guide the user to choose experiment settings such as sample groups, tubes, and pulse parameters for data acquisition. Once a template is selected, the user can start the acquisition or compensation wizard.
Data Analysis and Display
Data is displayed as dot plots and histograms on worksheets, and events can be marked using gates. The software has a number of tools to select, adjust, label, and measure statistics of target populations. Data can be easily exported as FCS formats (3.0 or 3.1 ) to use with third party analysis software.
Once events are gated on plots, targeted cells can be sorted for further analysis. Sort control is managed in a simple dialog where settings and configurations are selected. The SH800S Cell Sorter supports a variety of sorting methods and vessels including 2-way tubes, 8-well strips, slides, and 6-, 12-, 24-, 48-, 96- and 384-well plates.
The system supports eight sorting modes to provide different levels of purity (including single-cell mode) and yield to enable optimal results. In addition, the software provides an assisted mode for large cells to improve sorting performance. Three sorting chip sizes (70 μm, 100 μm and 130 μm) are available to accommodate a range of cell sizes and applications.
The BCC300AMS Class II biosafety cabinet custom designed for SH800S by the Baker Company is available as an option to provide personnel and product protection. The cabinet measures 118 cm (W) x 99.1 cm (D) x 224 cm (H).
Biosafety Safety Standards Compliance
The cabinet was tested by The Baker Company using microbiological assays with the SH800S Cell Sorter inside the work area. The testing concluded that the biosafety cabinet with the SH800S inside met several international biosafety standards including the National Sanitation Foundation Standard 49 (NSF49), and the European Standard 12469.
Built-in Aerosol Management
The cabinet incorporates a built-in aerosol management system which operates independently to actively evacuate aerosols from the sort collection chamber. The dual routes of aerosol evacuation ensure maximum personnel protection.
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SH800S Cell Sorter applications
Gates and Statistics
SH800S Cell Sorter specifications